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KMID : 0613820090190020169
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Journal of Life Science
2009 Volume.19 No. 2 p.169 ~ p.173
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Assessment of Genetic Diversity of Horse Breeds Using Microsatellite Makers
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Jung Ji-Hye
Lee Mi-Rang
Ha Tae-Yong
Kim Seon-Ku
Shin Taek-Soon
Kang Han-Seok
Lee Hong-Gu
Cho Gil-Jae
Park Kyung-Do
Cho Byung-Wook
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Abstract
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To assist in selection schemes we estimate the genetic diversity of the horse breeds. Genetic diversity at 13 microsatellite loci was compared in six horse breeds : Jeju Native Horse, American Quarter, Jeju Racing Horse, Mongolian Horse, Japanese Horse and Thoroughbred. All of the equine microsatellite used in this study were amplified and were polymorphic. The expected total heterozygosity over all the populations varied between 0.669 and 0.869 and the expected heterozygosity within population range from 0.569 to 0.219 in this study. The low coefficient of gene differentiation value showed that only 0.118 of the diversity was between horses breeds. The constructed dendrogram from the genetic distance matrix showed little differentiation between horse breeds using DISPAN program. The genetic distance using 13 microsatellites ranged between 0.137 and 0.414 for the six horse breeds. These results confirm the potential use of equine microsatellite loci as a tool for genetic studies in horse populations. The genetic diversity of the six horse breeds to each other closed to their geographical distribution. Suggesting that the loci would be suitable for horse breeds parentage testing. Therefore, Microsatellite marker seems to be very useful for clarifying the evolutionary relationships of closely related populations.
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KEYWORD
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Genotype, genetic diversity, microsatellite markers, horse breeds
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